Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link

Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed flavonoids contained composition. The species also a constituent of processed products are commercialized various parts the world including Brazil, although there few reports literature about development an analytical method for quantification it. main purpose this research was evaluation methodology determinate content total leaves S. occidentalis, quality control. parameters evaluated were: drug: solvent ratio, concentration aluminum chloride reaction time. obtained after with by UV/Vis spectrophotometry. results revealed maximum absorption peak (λ = 392 nm) amount 0.5 g plant raw 100 mL were no significant influences between concentrations or hydroalcoholic solutions analyzed. time allowed characterize kinetics slow, stabilizing from 60 min, choosing reading specific, linear, precise, accurate robust, according specifications set RDC n. 166/2017. Finally, study showed that measured simple can be applied assessment occidentalis.